Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Biochemical Research   Volumes    Volume 9  Issue 2
Abstract
Singlet oxygen generates biological peroxides
G. E. Ronsein, S. Miyamoto, M. H. G. Medeiros, P. Di Mascio
Pages: 79 - 91
Number of pages: 13
Current Topics in Biochemical Research
Volume 9  Issue 2

Copyright © 2007 Research Trends. All rights reserved

ABSTRACT
 
Singlet oxygen (1O2) is known to be produced by a number of enzymes as well as by UV or visible light in the presence of a sensitizer. It also can be formed during the dimerization reactions of peroxyl radicals in lipid peroxidation, via the Russell mechanism. Singlet oxygen shows considerable reactivity toward electron-rich organic molecules, leading to the formation of endoperoxides, dioxetanes or allylic hydroperoxides. Therefore, biomolecules such as lipids, proteins and DNA are important targets of 1O2. Lipid double bonds react with 1O2 by the ene reaction, yielding hydro-peroxides. In proteins, only cysteine, histidine, methionine, tryptophan, and tyrosine react significantly with 1O2 at neutral pH. For example, it was shown that 1O2 oxidation generates dioxetanes and/or hydroperoxides within the side chain residues of aromatic amino acids. Moreover, in DNA, guanine is the only nucleic acid component that exhibits significant reactivity toward 1O2 at neutral pH. One important product of this reaction is 8-oxo-7,8-dihydro-2’-deoxyguanosine, a mutagenic lesion. The peroxides formed by 1O2 oxidation and their decomposition products have been shown to give rise to further damage, leading to deleterious biological effects such as aging, neurodegenerative disease, atherosclerotic lesions, mutagenesis, and carcinogenesis. Recently, the development of new techniques, using 18O-labeled compounds and HPLC coupled to tandem mass spectrometry (HPLC-MS/MS), has provided insights to elucidate the mechanisms by which peroxides can increase initial damage in biological systems. In this work, we report some aspects of 1O2 oxidative damage to biomolecules and recent developments in the understanding of this type of damage, using techniques such as HPLC-MS/MS.
Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms