Lipoproteins are complexes of proteins and lipids, and have important roles of lipid transport through circulation of blood. Cultured eel hepatocytes actively synthesize and secrete chylomicron-like lipoprotein. We describe the regulation of the chylomicron-like lipoprotein synthesis and regulation as follows. 1. Characterization of the lipoprotein secreted by cultured eel hepatocytes. 2. Effects of hormones such as insulin, thyroxine, and estradiol. 3. Effects of eel serum very low density lipoprotein (VLDL) and high density lipoprotein (HDL). 4. Effects on docosahexaenoic acid (DHA).